Unveiling the mechanism of Pogostemon cablin (Blanco) Benth in treating heat illnesses via network pharmacology and molecular simulation.

ABSTRACT:

Background

Heat illness is a dangerous condition marked by a widespread inflammatory response. Although Pogostemon cablin (Blanco) Benth and its derivatives are clinically used, their mechanisms remain unclear.

Methods

11 heat illness patients and 14 healthy volunteers from Southwest Medical University Affiliated Hospital were enrolled. Bulk RNA sequencing of peripheral blood samples identified disease-relevant modules via weighted gene co-expression network analysis (WGCNA). Active ingredients and targets of P. cablin were retrieved from TCMSP. GO/KEGG, protein-protein interaction (PPI), and ROC analyses were performed. Core genes were localized through single-cell RNA sequencing, with compound-target interactions validated by molecular docking.

Results

Enrichment an

SUBMITTER: Li X 

PROVIDER: S-EPMC12416687 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature