A duplex real-time loop-mediated isothermal amplification assay for simultaneous detection of elephant endotheliotropic herpesvirus types 1 and 5.

ABSTRACT: Elephant Endotheliotropic Herpesviruses (EEHVs) are the cause of a highly fatal haemorrhagic disease (HD) in elephants primarily affecting young Asian elephants (Elephas maximus) in both captivity and in the wild. Timely and accurate detection of EEHVs is crucial not only for antiviral and supportive treatment, but also for the prevention and control of the EEHV pandemic with proper preventive intervention measures, which requires a simple and rapid assay. EEHVs contain seven genotypes (EEHV1-7), and EEHV1, EEHV4 and EEHV5 are mainly circulating in Asian elephants. Here, we developed a rapid, sensitive and specific duplex real-time LAMP assay for simultaneous detection of EEHV1 and EEHV5. The assay exhibited remarkable specificity with no cross reactions with nine other herpesviruse

SUBMITTER: Xu C 

PROVIDER: S-EPMC12417173 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature