Enhanced sensitivity detection via orientational labelling of SPA-functionalized magnetic nanoparticles in lateral-flow immunoassays.

ABSTRACT: Mycoplasma pneumonia, a primary aetiological agent of atypical pneumonia, necessitates the implementation of rapid point-of-care diagnostics. Lateral flow immunoassays (LFIAs) hold promise for point-of-care testing (POCT), yet their sensitivity levels are frequently constrained by probe affinity and matrix interference. We introduce an orientational labelling strategy that employs magnetic nanoparticles (MNPs) functionalized with staphylococcal protein A (SPA) to simultaneously enhance antibody orientation and facilitate magnetic enrichment. The SPA-MNPs were synthesized via aggregation‒precipitation crosslinking, utilizing the Fc-binding specificity of SPA to enable oriented antibody conjugation and reduce steric hindrance. The oriented probe demonstrated robust stability, exceptional spe

SUBMITTER: Zhao P 

PROVIDER: S-EPMC12417246 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature