The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.

ABSTRACT: Copy number control of DNA and centrosomes is essential for accurate genetic inheritance. DNA replication and centrosome duplication have been recognized as parallel key events for cell division. Here, we discover that the DNA replication machinery directly regulates the licensing and execution processes of centrosome duplication to prevent centrosome amplification. We find that the microcephaly protein DONSON couples DNA replication initiation with Cdc6 translocation to centrosomes. The Cdc6 signal prevents the precocious occurrence of centriole disengagement, the licensing step for centrosome duplication. During DNA replication, DONSON inhibits replisome disassembly by interacting with the CMG helicase, maintaining the intrinsic S/G2 checkpoint signal that blocks centriole-to-centrosome

SUBMITTER: Matsuhashi K 

PROVIDER: S-EPMC12417547 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature