Enantioselective Properties of Neglected Drug Nifurtimox on Polysaccharide and Macrocyclic Glycopeptide Chiral Stationary Phases by Green HPLC Separation Methods.

ABSTRACT: Nifurtimox (NFX) is a chiral drug used for the treatment of Chagas Disease. Little attention has been paid to the enantioselective properties of chiral drugs used for neglected tropical diseases, highlighting the need for further studies in this area. In this work, the enantioselective properties of NFX were carefully investigated by HPLC using different chiral stationary phases (CSPs) and chromatographic modes. Two enantioseparation HPLC methods were successfully developed and validated. Polar ionic, polar organic, and RP modes were tested, and the polar organic mode proved to be suitable for the enantioseparation. Chromatographic resolution was achieved using the polysaccharide-based Chiralpak AD CSP and the macrocyclic glycopeptide-based Chirobiotic V CSP. Both methods employed ethanol

SUBMITTER: Mohr A 

PROVIDER: S-EPMC12417557 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature