A Surface Plasmon Resonance-Based Integrated Assay for Quantification and Glycosylation Characterization of Monoclonal Antibodies in Crude Heterogeneous Samples.

ABSTRACT: The rise in cancer, autoimmune, inflammatory, and infectious diseases in recent decades has led to a surge in the development of monoclonal antibodies (mAbs) therapies, now the most widely used family of biologics. To meet the growing global demand, biopharmaceutical industries are intensifying their production processes. One approach to achieve more efficient production of effective mAbs is to develop tools for real-time quality monitoring. Specifically, the glycosylation profile of mAbs must be closely monitored, since it greatly impacts their therapeutic efficacy and innocuity, making it a critical quality attribute. In this study, we developed a surface plasmon resonance-based integrated assay allowing for the simultaneous quantification and glycosylation characterization of mAbs in cr

SUBMITTER: Metayer I 

PROVIDER: S-EPMC12417749 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature