Geometrically Controlled WNT Activation Drives Intestinal Morphogenesis.

ABSTRACT: Morphogenesis is a multifaceted process that integrates biochemical signals with mechanical and architectural cues to drive tissue formation. Here, the modulation of WNT signaling and engineered microenvironments synergistically drive crypt-villus-like morphogenesis in colorectal carcinoma (Caco-2) cells. Fibroblast conditioned media induced WNT-dependent budding, confirmed via secretome profiling and WNT inhibition by Dickkopf-1 (DKK1). Direct modulation of WNT activity with agonist CHIR enhanced both epithelial budding and mucin 2 (MUC2) expression. To isolate the role of architecture in this process, fabricated gelatin methacrylate (GelMA) microwell arrays via digital light processing (DLP) printing enabled independent control of geometry and stiffness. Increased scaffold perimeter-to-a

SUBMITTER: Burmas NC 

PROVIDER: S-EPMC12417773 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature