Thermoresponsive BrushGel Microcarriers for Efficient Cell Expansion and Enzyme-Reduced Harvesting.

ABSTRACT: Scaling up cell therapy requires efficient expansion of high-quality cells. Microcarrier(MC)-based systems offer high surface-to-volume ratios and reduce culture media usage. In this study, we developed BrushGel, a temperature-responsive MC composed of gelatin methacryloyl (GelMA) hydrogel particles coated with poly(N-isopropyl acrylamide) (PNIPAM) polymer brushes via covalent grafting. BrushGel was fabricated using a flow-focusing droplet microfluidic device and functionalized using carbodiimide chemistry ( 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide-N-Hydroxysuccinimide, EDC-NHS). The degree of PNIPAM coating was tuned by varying the degree of methacrylation (DOM) of GelMA and the concentration of PNIPAM. Human dermal fibroblast (HNDF) cells cultured on the BrushGel under dynamic condi

SUBMITTER: Askari E 

PROVIDER: S-EPMC12417781 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature