Scalable, High-Density Expansion of Human Mesenchymal Stem Cells on Microcarriers Using the Bach Impeller in Stirred-Tank Reactors.

ABSTRACT: This paper describes the results of process developmental experiments to achieve higher cell densities in the manufacturing of hMSCs using the novel Bach impeller in a stirred-tank bioreactor. Engineering experiments have previously shown that the Bach impeller represents an efficient mixing device that suspends particles in fluids at low power inputs. To assess the impeller during biological experiments, the growth performance of Wharton Jelly (WJ)-hMSCs in a 1 L STR equipped with the Bach impeller was evaluated at a variety of culture conditions. The cells attached to Cytodex 1 microcarriers at a concentration of 5.6 g/L and were cultured for 5-7 days. The growth phase was carried out at varying impeller speeds N  = 75, 115, and 150 rpm. Cell growth was additionally evaluated at a microc

SUBMITTER: Wyrobnik TA 

PROVIDER: S-EPMC12417783 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature