The proteomic landscape of diffuse midline glioma highlights the therapeutic potential of non-histone protein methyltransferases.

ABSTRACT:

Background

Diffuse midline glioma (DMG) is a highly aggressive pediatric brain tumor with limited treatment options despite extensive genomic characterization. The aim of this study was to investigate the proteomic landscape of DMG to identify potential therapeutic targets.

Methods

We conducted a comprehensive proteomic analysis using LC-MS3, along with DNA methylation and DNA/RNA sequencing in 55 DMG patients' samples. post-translational modification profiling (phosphoproteome and methylproteome) was conducted in 30 patient samples. We then investigated the effects of modulating key protein targets on protein methylation, protein synthesis, and DMG cell growth in vitro and in vivo.

Results

DMGs exhibited high global protein methylation, with significant enrichment of

SUBMITTER: Anguraj Vadivel AK 

PROVIDER: S-EPMC12417832 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature