Effects of Short-Term Calcifediol Supplementation on Leukocyte Cytokine Production in Healthy Dogs: A Randomized, Double-Blinded, Placebo-Controlled Crossover Trial.

ABSTRACT:

Background

Vitamin D modulates the immune response in many species, including dogs. To date, research investigating the immunological effects of vitamin D in dogs is limited to in vitro studies.

Objectives

Provide PO calcifediol supplementation to healthy dogs to evaluate its tolerability and assess its effect on leukocyte production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10.

Animals

Eleven healthy client-owned dogs with serum 25-hydroxyvitamin-D₃ (25(OH)D₃) concentrations ≤ 30 ng/mL.

Methods

Prospective, randomized, double-blinded, placebo-controlled crossover study. Dogs were randomized to receive calcifediol at 2.3 μg/kg^0.75 (low-dose), 4.6 μg/kg^0.75 (high-dose), or placebo for 7 days and cros

SUBMITTER: Jaffey JA 

PROVIDER: S-EPMC12418335 | biostudies-literature | 2025 Sep-Oct

REPOSITORIES: biostudies-literature