Retinoic acid-inducible gene-I aggravates neuroinflammation in early brain injury after subarachnoid hemorrhage through mediating brain microvascular endothelial cell pyroptosis.

ABSTRACT: Retinoic acid-inducible gene-I (RIG-I) is an immune signal that promotes inflammatory responses and plays an important role in endothelial cell-mediated inflammation. Currently, no studies have investigated the role of RIG-I in early brain injury (EBI) after subarachnoid hemorrhage (SAH). In this research, an in vivo SAH model was established in Sprague Dawley (SD) rats through carotid artery puncture, while oxyhemoglobin (OxyHb) was used to stimulate brain microvascular endothelial cells (BMVECs) to generate an in vitro SAH model. The results showed that RIG-I was activated and expressed in BMVECs in both in vivo and in vitro. To explore how RIG-I is involved in the EBI, small interfering RNA was used to downregulate its expression. Compared with SAH rats, RIG-I knockdown rats had better

SUBMITTER: Sun B 

PROVIDER: S-EPMC12418424 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature