A Method for Comparing Proteins Measured in Serum and Plasma by Olink Proximity Extension Assay.

ABSTRACT: Accurate measurement of secreted proteins in serum and plasma is essential for understanding mechanisms and developing reliable biomarkers. Recent technological advancements, such as proximity extension assay (PEA), have enabled high-throughput multiplex protein analyses from small sample volumes in either serum or plasma. Despite the increasing use of PEA-based proteomics and the generation of extensive datasets, integrated data from these two mediums remains challenging due to inherent differences in sample processing. To address this issue, we developed and validated protein-specific transformation factors using linear modeling to normalize protein measurements between serum and plasma proteins quantified using Olink. Our analysis surveyed 1463 proteins across matched serum and plasma s

SUBMITTER: Shraim R 

PROVIDER: S-EPMC12418474 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature