The Identification by Shotgun Proteomics with High-Resolution Tandem Mass-Spectrometry of Histone Isoforms' Hypermethylation Phenotype as a Hallmark Characteristic of Human-IDH-Mutant High-Grade Gliomas: Epigenetic Applications for Genotoxicity-Based Biomarkers and Cancer Therapy Targets.

ABSTRACT: Histone post-translational modifications (PTMs) have been linked to various pathological processes, especially in cancer onset, where they are envisaged as obvious diagnostic biomarkers and pivotal predictors for pathological prognosis. Consequently, their mapping and characterization constitute a critical field of study facilitated by recent advances in the high-throughput mass spectrometry technique. The current study aimed to clarify the neurotoxicity mechanisms at the epigenetic level induced by environmental stressors by examining their potential to induce aberrant histone methylation as it is the most involved modification in carcinogenesis. Our protocol first consisted of a 3D in vitro neurospheroid model derived from human high-grade gliomas, followed by treatment with a pes

SUBMITTER: Louati K 

PROVIDER: S-EPMC12418497 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature