Applying a Conservation-Based Approach for Predicting Novel Phosphorylation Sites in Eukaryotes and Evaluating Their Functional Relevance.

ABSTRACT: Protein phosphorylation, a key post-translational modification, is central to cellular signaling and disease pathogenesis. The development of high-throughput proteomics pipelines has led to the discovery of large numbers of phosphorylated protein motifs and sites (phosphosites) across many eukaryotic species. However, the majority of phosphosites are reported from human samples, with most species having a few experimentally confirmed or computationally predicted phosphosites. Furthermore, only a small fraction of the characterized human phosphoproteome has an annotated functional role. A common way of predicting functional phosphosites is through conservation-based sequence analysis, but large-scale evolutionary studies are scarce. In this study, we explore the conservation of 20,751 confi

SUBMITTER: Kalyuzhnyy A 

PROVIDER: S-EPMC12418505 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature