Sex Identification and Species Confirmation in Modern and Archeological Caprine Enamel.

ABSTRACT: Proteomics has become a transformative tool for species and sex determination. This study introduces a novel methodology that integrates amelogenin (Amel) and enamelin (Enam) proteins extracted from the tooth enamel of caprines. Since morphologically, osteological remains of sheep and goats often cannot be easily discriminated, we developed our method on both modern domestic sheep (Ovis aries) and goats (Capra hircus) to establish unique proteomic signatures for each species for sex and species identification. Applying a targeted parallel reaction monitoring (PRM) assay, we validated the sex and species of 8 modern domestic sheep and 6 domestic goats. We then applied the same method to 10 ancient samples dating to the early eighth millennium BC Neolithic period. For sex deter

SUBMITTER: Kotli P 

PROVIDER: S-EPMC12418508 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature