Epigenetic modulation of Ceratorhiza hydrophila by 5-azacytidine enhances antifungal metabolite production: insights from antimicrobial, metabolic, genomic and computational analyses.

ABSTRACT:

Background

The emergence of drug-resistant pathogens has stimulated the need for the development of new antimicrobial agents. Epigenetic modulation by suppressing epigenetic inhibitors, such as 5-azacytidine (5-aza), has been shown to activate silent biosynthetic gene clusters within a fungus and causes the production of novel secondary metabolites. This research examined this epigenetic modification strategy in the poorly studied filamentous fungus, Ceratorhiza hydrophila, which may help induce the additional production of bioactive compounds.

Results

The results from genomic and spectroscopic analyses (ISSR profiling and FTIR spectroscopy) indicated that 50 µM 5-aza produced substantial global DNA demethylation and genomic changes in C. hydrophila with no impact on cell vi

SUBMITTER: Abdelhamid RM 

PROVIDER: S-EPMC12418614 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature