Stochastic to Deterministic: A Straightforward Approach to Create Serially Perfusable Multiscale Capillary Beds.

ABSTRACT: Generation of in vitro tissue models with serially perfused hierarchical vasculature would allow greater control of fluid perfusion throughout the network and enable direct mechanistic investigation of vasculogenesis, angiogenesis, and vascular remodeling. In this work, we have developed a method to produce a closed, serially perfused, multiscale vessel network fully embedded within an acellular hydrogel, where flow through the capillary bed is required prior to fluid exit. We confirmed that the acellular and cellular gel-gel interface was functionally annealed without preventing or biasing cell migration and endothelial self-assembly. Multiscale connectivity of the vessel network was validated via high-resolution microscopy techniques to confirm anastomosis between self-assembled a

SUBMITTER: Donzanti MJ 

PROVIDER: S-EPMC12418680 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature