Exploring the protective role of DDIT4/mTOR in podocyte integrity through macrophage polarization in diabetic kidney disease.

ABSTRACT:

Objectives

In this study, we explored the mechanism by which DDIT4 influences the polarization phenotypic transformation of macrophages and inflammation through the regulation of mTOR signaling pathway, providing a new mechanism and target for the treatment of diabetic nephropathy.

Methods

The degree of inflammation and injury in renal tissues of diabetic kidney disease (DKD) animal model was evaluated using biochemical assays, renal pathology examinations, and Western blot tests. Podocytes and macrophages were isolated from renal tissues to observe the extent of podocyte injury and the quantity and polarization phenotype of macrophage infiltration. Subsequently, the activated M1 macrophage model was constructed and transfected with DDIT4 to evaluate the effect of DDIT4 on t

SUBMITTER: Shi J 

PROVIDER: S-EPMC12418801 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature