Biocatalytic cascade combining an engineered pyranose 2-oxidase and transaminases for the synthesis of amino sugars.

ABSTRACT: This work reports cascade reactions using a pyranose 2-oxidase and transaminases for synthesizing the rare amino sugars mannosamine, glucosamine, and galactosamine. Transaminases were engineered to accept unnatural 2-keto-sugars, providing up to 68% galactosamine yield. The mechanisms of C2-amination by these transaminases were also investigated. These transaminase variants have potential applications in high-value amino sugar synthesis.

SUBMITTER: Chuaboon L 

PROVIDER: S-EPMC12418926 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature