Spatial proteomics reveals distinct protein patterns in cortical migration disorders caused by LIN28A overexpression and WNT activation.

ABSTRACT: Developmental signalling pathways act in stage and tissue dependent relation and mis-activation can drive tumour formation. The RNA-binding protein LIN28A binds to mRNA and miRNA and thereby affects the protein turnover and maintains stemness. LIN28A is overexpressed in embryonal brain tumours which show low correlation between transcriptome and proteome signatures. Additionally, stabilising CTNNB1 mutations activating the WNT pathway have been reported in brain tumours with LIN28A overexpression. The aim of this study was to co-activate these oncogenic proteins during embryonal brain development and investigate the histomorphology of the cerebral cortex in relation to proteome levels with spatial resolution using the nanosecond infrared laser (NIRL) system for nano-volume sampling. The co

SUBMITTER: Navolic J 

PROVIDER: S-EPMC12419088 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature