Discovery of Novel Hydroxyproline-Containing Microcystins in Western Lake Erie Cyanobacterial Bloom Samples.

ABSTRACT: From midsummer to fall, cyanobacterial harmful algal blooms (cHABs) producing microcystins (MCs), a class of hepatotoxic cyclic heptapeptides, plague Lake Erie's western basin. Since MC congener toxicities vary over 2 orders of magnitude, identification and characterization of the microcystin congener profile can be crucial information when balancing between public health and local economic resilience. Herein, five hydroxyproline (Hyp)-containing MCs were discovered in a Lake Erie cHAB. High-resolution liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS), tandem mass spectrometry (MS/MS), and thiol derivatization were used in the putative identification of novel MC congeners. Among the five novel congeners, MC-LHyp, MC-HpeHyp, [Dhb⁷]MC-HilHyp, and [Dhb

SUBMITTER: Premathilaka SH 

PROVIDER: S-EPMC12419242 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature