Genomic features and fitness cost of co-existence of <i>bla</i> _KPC-2 and <i>bla</i> _VIM-2 plasmids in ICU-derived pan-drug resistant <i>Pseudomonas aeruginosa</i>.

ABSTRACT:

Backgroud

The emergence of carbapenem-resistant Pseudomonas aeruginosa (CRPA) co-producing KPC-2 and VIM-2 has increased the healthcare threats.

Results

In this study, a CRPA strain 18102011, was isolated from the bile of a burn patient in ICU of China. Its whole genome was sequenced via the PacBio platform. The molecular characteristics of the genome were analyzed to assess the genetic environment of the carbapenemase genes bla _KPC-2 and bla _VIM-2. Antimicrobial susceptibility, plasmid stability, bacterial growth curves, and plasmid conjugation were measured. Strain 18102011 exhibited a resistant pattern to all 23 antibiotics tested, which could be defined as a pan-drug resistant P. aeruginosa strain. Two plasmids were ident

SUBMITTER: Zheng L 

PROVIDER: S-EPMC12420198 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature