Proteomic profiling reveals dynamic regulation of vesicle trafficking across glioma grades.

ABSTRACT:

Purpose

Gliomas are highly heterogeneous central nervous system tumors that evolve through progressive molecular reprogramming. While cell proliferation and adhesion mechanisms are well-characterized, the contribution of vesicle trafficking to glioma progression remains underexplored. This study aimed to characterize proteomic changes across glioma grades.

Methods

We performed untargeted, quantitative proteomic profiling of glioma tissues across WHO grades I-IV using a combination of Tandem Mass Tag (TMT)-11plex labeling and high-resolution liquid chromatography-mass spectrometry (LC-MS). Tissue samples were processed using filter-aided sample preparation (FASP) and analyzed using a µPAC reverse-phase HPLC system coupled to a high-resolution mass spectrometer. Protein identi

SUBMITTER: Pienkowski T 

PROVIDER: S-EPMC12420692 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature