Evaluation of canine epidermis and keratinocytoids (keratinocytic organoids) by transmission electron microscopy.

ABSTRACT:

Background

Skin disease is a common complaint in veterinary medicine. Current models often use live animals. Epidermal organoids (keratinocytoids) are 3D miniature organs created in culture from single epidermal keratinocytes. These keratinocytoids can be used as an alternative to live animal studies to investigate epidermal structures and skin diseases.

Hypothesis/objective

This study's objective was to compare ultrastructural morphological features of canine keratinocytoids to those features of normal epidermis.

Results

Skin and organoids had morphologically similar components, including tight junctions, desmosomes, lamellar bodies and keratin filaments. These structures were similar in both morphology and distribution. Keratinocytes in the organoids had larger and

SUBMITTER: Lui CC 

PROVIDER: S-EPMC12420878 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature