Mechanisms of c-Fos regulation of mTOR signaling via ERα/β in abnormal lipid metabolism of granulosa cells in PCOS.

ABSTRACT:

Objective

This study investigates the role of the c-Fos/estrogen receptors (ERs)/mTOR pathway in lipid metabolism in human follicular granulosa cells of individuals with polycystic ovary syndrome (PCOS). Specifically, we aim to determine whether c-Fos targets estrogen receptors (ERα and ERβ) to mediate the mTOR pathway, influencing lipid metabolism, and to identify the key molecular mechanisms involved.

Methods

A PCOS mouse model was established using dehydroepiandrosterone (DHEA), and ovarian tissues were collected from both PCOS and control mice. RT-qPCR and Western blotting were used to measure the expression levels of c-Fos, ERα, ERβ, and mTOR. Follicular fluids were obtained from patients with PCOS and male factor infertility on the day of ovulation. Adenovirus-mediated

SUBMITTER: Chen X 

PROVIDER: S-EPMC12421171 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature