SyFi: generating and using sequence fingerprints to distinguish SynCom isolates.

ABSTRACT: The plant root microbiome is a complex community shaped by interactions among bacteria, the plant host and the environment. Synthetic community (SynCom) experiments help disentangle these interactions by inoculating host plants with a representative set of culturable microbial isolates from the natural root microbiome. Studying these simplified communities provides valuable insights into microbiome assembly and function. However, as SynComs become increasingly complex to better represent natural communities, bioinformatics challenges arise. Specifically, accurately identifying and quantifying SynCom members based on, for example, 16S rRNA amplicon sequencing becomes more difficult due to the high similarity of the target amplicon, limiting downstream interpretations. Here, we presen

SUBMITTER: Selten G 

PROVIDER: S-EPMC12421257 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature