Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches.

ABSTRACT: Effective tracheal tissue engineering benefits from scaffolds that mimic the native structure of the tissue, provide mechanical stability, and support spatially controlled cell seeding to encourage tissue regeneration. This study presents a novel approach for fabricating tubular scaffolds for tracheal regeneration that integrates a 3D-printed polycaprolactone (PCL) backbone with a freeze-dried collagen-hyaluronic acid (CHyA) layer. Two scaffold geometries (tubular and c-shaped) were produced and mechanically characterized, and it was demonstrated that PCL reinforcement significantly enhanced scaffold structural robustness and durability. To achieve spatially selective cell seeding, custom-designed PLA accessories facilitated the precise deposition of respiratory epithelial cells (Calu-3) o

SUBMITTER: Soriano L 

PROVIDER: S-EPMC12421502 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature