Strong Association between Proanthocyanidins and Polysaccharides in the Cell Walls of Western Redcedar Bark.

ABSTRACT: The co-occurrence of polysaccharides and proanthocyanidins in the aqueous extracts of western redcedar (Thuja plicata Donn; WRC) bark limits their commercial utilization. To better understand their association, proanthocyanidins and polysaccharides were extracted with cold water (3.4% w/w bark) and isolated as an alcohol-insoluble residue (AIR, 1.0% w/w bark). The polysaccharide content (∼30% w/w AIR) was analyzed by acidic and enzymatic depolymerization, revealing the presence of pectins, xyloglucans, and xylans. NMR spectroscopy identified features, such as acetylation and methyl esterification. Thiolysis followed by HPLC-DAD revealed that proanthocyanidins (1.46% w/w AIR) exhibit a mean degree of polymerization of 5.3, a cis/trans ratio of 0.40, and a procyanidin/pr

SUBMITTER: Bautista GFM 

PROVIDER: S-EPMC12421503 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature