Fluorinated Redox-Responsive Cross-Linked Poly(amidoamine) G2 as Smart Theranostic Dendrimers.

ABSTRACT: This work presents a novel strategy for enhancing the gene delivery capabilities of low-generation poly(amidoamine) (PAMAM) dendrimers by employing a fluorinated, redox-responsive cross-linker. The synthesized cross-linker, featuring both disulfide bonds and trifluoromethyl groups, facilitates efficient "click" Michael addition with PAMAM G2, producing cross-linked polymers with strong ¹⁹F nuclear magnetic resonance signals and favorable relaxation properties, enabling their use as ¹⁹F nuclear magnetic resonance tracers. The resulting fluorinated PAMAM G2 polymers also demonstrated excellent plasmid DNA complexation, glutathione-triggered degradation, and notably enhanced transfection efficiency, especially in cancerous HeLa cells notoriously associated with a reducin

SUBMITTER: Romani C 

PROVIDER: S-EPMC12421505 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature