Modification of Bacterial Nanocellulose Using Nonthermal Plasma-Assisted Enzymatic Hydrolysis.

ABSTRACT: This study explored the structural modification of bacterial cellulose (BC) through enzymatic hydrolysis using varying cellulase activities and substrate concentrations. Optimal hydrolysis conditions (50 U/g of BC; 20 g/L of BC) were established to balance recovery and homogeneity (yielding BNC1). Hydrolysis was further combined with nonthermal plasma by suspending BC into plasma-activated water (PAW) prior to hydrolysis (BNC2). In another approach, BC suspensions were pretreated using a plasma bubble reactor followed by hydrolysis (BNC3). BNC1 and BNC2 yields were similar (∼50%), suggesting that PAW regulated the pH during hydrolysis. BNC3 yield was significantly higher (78%) compared to BNC1, indicating that the generated radicals promoted chain modifications while minimizing glucose/cel

SUBMITTER: Sarafidou M 

PROVIDER: S-EPMC12421519 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature