Matrix-independent screening of defluorination <i>in vitro</i> and <i>in vivo</i>.

ABSTRACT: There is intense interest in biodegrading fluorinated pesticides and other commercial products, some of which are per- and poly-fluorinated alkyl substances, or PFAS. Enzymatic carbon-fluorine bond cleavage via hydrolytic, reductive, and eliminative mechanisms generates an organic product, fluoride anion, and a proton. Biodegradation is typically determined by tracking the organic product using liquid chromatography-mass spectrometry (LC-MS) or the anion determined by a fluoride-specific electrode. Here, we monitored the protons that are produced. A pH indicator method was developed using a hydrolytic defluorinase from Delftia acidovorans strain B in purified form or expressed recombinantly in Pseudomonas putida ATCC 12633. The method was also shown to be effective with P.

SUBMITTER: Simon AT 

PROVIDER: S-EPMC12421806 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature