Loss of cardiolipin and porins bypasses the essentiality of the sigma E cell envelope stress response in <i>Escherichia coli</i>.

ABSTRACT: The Gram-negative cell envelope is composed of an inner and outer membrane, each with different lipid compositions. The inner membrane is predominantly made up of phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin, while the outer membrane contains the same lipids but has a predominantly lipopolysaccharide composition in the outer leaflet. The outer membrane forms a permeability barrier that blocks the entry of toxins and antibiotics; however, it is decorated with outer membrane proteins that allow nutrient uptake and the efflux of intracellular toxins. In Escherichia coli, the σ^E stress response is essential for viability but is upregulated when disruptions to outer membrane protein biogenesis occurs. Here, we used a high-throughput genetic screen to identif

SUBMITTER: Yang Z 

PROVIDER: S-EPMC12421810 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature