Synthetic C₁ metabolism in <i>Pseudomonas putida</i> enables strict formatotrophy and methylotrophy via the reductive glycine pathway.

ABSTRACT: Formate and methanol are promising alternatives to sugar-based feedstocks for biotechnological applications. These one-carbon (C₁) substrates can be sustainably produced from CO₂ and renewable electricity and assimilated by both native and engineered microbial systems. However, their broader adoption is limited by the narrow range of bacterial hosts capable of efficient methanol and formate utilization. In this study, the industrially relevant soil bacterium Pseudomonas putida was metabolically engineered to assimilate formate and methanol as sole carbon and energy sources via the linear reductive glycine pathway. Initial strains were optimized for formate assimilation using acetate for energy conservation through adaptive laboratory evolution (ALE), leading to

SUBMITTER: Turlin J 

PROVIDER: S-EPMC12421819 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature