Encephalomyocarditis virus protein 2B* interacts with 14-3-3 proteins through a phosphorylated C-terminal binding motif.

ABSTRACT: Encephalomyocarditis virus (EMCV) has been an important model RNA virus for decades. Although most of the EMCV proteins are obtained via proteolytic cleavage of a long polyprotein, 2B* is expressed from a short overlapping open reading frame via an unusual protein-stimulated temporally dependent ribosomal frameshifting mechanism. Mutant viruses that are unable to express 2B* have a small plaque phenotype due to delayed onset and inefficient progression of multiple lytic cell death pathways. However, the mechanism by which 2B* promotes these pathways has not yet been characterized. By tagging 2B* within the viral genome, we identified putative interaction partners of 2B* and showed that 2B* binds all seven members of the 14-3-3 protein family during virus infection. Binding is entirely depe

SUBMITTER: Nguyen SK 

PROVIDER: S-EPMC12421828 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature