Conservation and evolution of the programmed ribosomal frameshift in <i>prfB</i> across the bacterial domain.

ABSTRACT: When the ribosome reaches a stop codon, translation is terminated by a release factor. Bacteria encode two release factors, RF1 and RF2. In many bacteria, the gene encoding RF2 (prfB) contains an in-frame premature stop codon near the beginning of the open reading frame. A programmed ribosomal frameshift is therefore required to translate full-length RF2. While the molecular mechanism of the programmed ribosomal frameshift has been extensively characterized in Escherichia coli, bioinformatic analysis of the evolution and conservation of this motif has been limited to few genomes. By analyzing >12,000 bacterial genomes, we sought to thoroughly characterize the conserved frameshifting elements within the programmed frameshift motif and identify genomic features of phyla that ha

SUBMITTER: Prince CR 

PROVIDER: S-EPMC12421855 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature