Hi-C untangles the temporal dynamics of the children's gut resistome and mobilome, highlighting the role of transposable elements.

ABSTRACT: Many metagenomic studies lack the ability to measure the temporal dynamics of the intestinal resistome (the collection of antibiotic resistance genes [ARGs]) and mobilome (the collection of all mobile genetic elements that enable their transfer) and link the genetic features to specific species in the gut. We applied Hi-C sequencing and shotgun metagenomics to study fecal matter from children (n = 15) living in semi-rural communities of Quito, Ecuador. We sampled at three different periods, with a 4- to 6-month interval between each sample collection. To understand the dynamics of ARGs from different genetic perspectives, we focused on identifying classes of mobile ARGs that are classified as high risk to human health. We selected those ARGs that appeared at least twice across sampl

SUBMITTER: Cifuentes SG 

PROVIDER: S-EPMC12421864 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature