Role of listeriolysin O and phospholipases C in <i>L. monocytogenes</i> intercellular protrusion dynamics, resolution, and autophagy avoidance.

ABSTRACT: Cell-to-cell spread is a major mechanism used by the bacterial pathogen Listeria monocytogenes to disseminate within its host. In this mechanism, bacteria are directly transferred from the cytosol of an infected donor cell to a recipient cell via formation of an intercellular protrusion. The intercellular protrusion resolves into a vacuole that is disrupted by L. monocytogenes to reach the cytosol of the recipient cell, where it divides and starts new cell-to-cell spread cycles. Here, we studied the spatiotemporal dynamics of the donor and recipient host cell plasma membranes during intercellular protrusion formation, resolution, and disruption, as well as the role of the bacterial pore-forming toxin listeriolysin O (LLO) and two phospholipases C in these dynamics. Leveraging

SUBMITTER: Fields M 

PROVIDER: S-EPMC12421892 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature