Osteogenic Small Molecule FK506 for Enhanced Bone Formation in a Rabbit Ankle Fusion Model.

ABSTRACT: Non-union remains a significant complication following ankle fusion procedures, underscoring the need for effective osteogenic adjuvants to improve boney healing and fusion outcomes. This study investigates the osteogenic potential of FK506 (Tacrolimus), both in vitro and in vivo, for enhanced ankle fusion. Known orthobiologics in the market, such as recombinant human platelet-derived growth factor-BB (rhPDGF-BB), have been utilized to promote bone healing and were included in this study to assess their comparative and combinatory effects with FK506. Bovine marrow-derived cells (MDCs) were cultured in 2D and 3D environments to assess temporal markers of osteogenesis: RUNX2 nuclear localization (early), Alizarin Red S staining (intermediate), and mineral deposition with micro-computed tomog

SUBMITTER: Huffman N 

PROVIDER: S-EPMC12422209 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature