Targeted gene silencing in mouse testicular Sertoli and Leydig cells using adeno-associated virus vectors.

ABSTRACT:

Abstract

Researchers commonly use cyclization recombination enzyme/locus of X-over P1 (Cre/loxP) technology-based conditional gene knockouts of model mice to investigate the functional roles of genes of interest in Sertoli and Leydig cells within the testis. However, the shortcomings of these genetic tools include high costs, lengthy experimental periods, and limited accessibility for researchers. Therefore, exploring alternative gene silencing techniques is of great practical value. In this study, we employed adeno-associated virus (AAV) as a vector for gene silencing in Sertoli and Leydig cells. Our findings demonstrated that AAV serotypes 1, 8, and 9 exhibited high infection efficiency in both types of testis cells. Importantly, we discovered that all three AAV serotypes exhibit

SUBMITTER: Pang J 

PROVIDER: S-EPMC12422574 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature