NK cell-related genes-driven novel molecular subtyping and prognostic signatures for Wilms tumor: uncovering the therapeutic potential of TGX-221 and biomarker role of HS2ST1.

ABSTRACT:

Background

Wilms tumor (WT) lacks precise molecular subtyping tools, which limits the development of personalized therapies. To address this issue, we investigated whether NK cell-related genes (NKGs) could refine the molecular subtyping of WT, aiming to identify novel therapeutic strategies.

Methods

Consensus clustering was employed for the molecular subtyping of WT. The immune microenvironment of different WT subtypes was assessed using immune profiling algorithms. Potential therapeutic compounds targeting the identified subtypes were screened using the CMap database, and their mechanisms of action were elucidated through molecular docking and molecular dynamics simulations. Subsequently, in vitro cell experiments, including CCK8, flow cytometry, and Transwell assay

SUBMITTER: Hong P 

PROVIDER: S-EPMC12422919 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature