Preclinical evaluation of 6-Gingerol in modulating gut microbiota and SCFAs to mitigate <i>Clostridium difficile</i>-associated diarrhea in mice.

ABSTRACT: Clostridium difficile associated diarrhea (CDAD) is a growing healthcare concern with limited effective treatments. 6-Gingerol, a major bioactive compound in ginger, exhibits notable antibacterial and anti-inflammatory properties, making it a potential alternative therapy. This study combines in vitro and in vivo approaches to evaluate its efficacy against CDAD. In vitro assays determined the half-maximal inhibitory concentration (IC₅₀) and minimum inhibitory concentration (MIC) of 6-Gingerol against C. difficile, which were 61.99 μM and 173.3 μM, respectively, indicating direct antibacterial activity. In vivo, a mouse model of CDAD was used to assess the therapeutic effects of 6-Gingerol. Outcomes included clinical symptoms, C. diffi

SUBMITTER: Ma FZ 

PROVIDER: S-EPMC12423060 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature