Constructing synthetic nuclear architectures via transcriptional condensates in a DNA protonucleus.

ABSTRACT: Nuclear biomolecular condensates are essential sub-compartments within the cell nucleus and play key roles in transcription and RNA processing. Bottom-up construction of nuclear architectures in synthetic settings is non-trivial but vital for understanding the mechanisms of condensates in real cellular systems. Here, we present a facile and versatile synthetic DNA protonucleus (PN) platform that facilitates localized transcription of branched RNA motifs with kissing loops (KLs) for subsequent condensation into complex condensate architectures. We identify salinity, monomer feeding, and KL-PN interactions as key parameters to control co-transcriptional condensation of these KLs into diverse artificial nuclear patterns, including single and multiple condensates, interface condensates, and bi

SUBMITTER: Xie M 

PROVIDER: S-EPMC12423304 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature