Circ_0074158 and QKI6: A regulatory axis for PD-L1 ubiquitination and immune evasion in NSCLC.

ABSTRACT:

Objective

CircRNAs are involved in cancer progression. However, their role in immune escape in non-small cell lung cancer (NSCLC) remains poorly understood.

Methods

This study employed RIP-seq for the targeted enrichment of circRNAs, followed by Western blotting and RT-qPCR to confirm their expression. Specific binding was assessed using electrophoretic mobility shift assays. Transwell and wound healing assays were performed to evaluate the invasive and migratory capacities of NSCLC cells. The secretion of TNF-α, IFN-γ, GzmB, and perforin by CD8⁺ T cells co-cultured with peripheral blood mononuclear cells (PBMCs) was quantitatively measured using enzyme-linked immunosorbent assays. The PD-L1 phenotype and apoptosis levels were determined by flow cytometry, while c

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12423385 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature