Repopulating Microglia Suppress Peripheral Immune Cell Infiltration to Promote Poststroke Recovery.

ABSTRACT:

Aims

Sustained neuroinflammation following ischemic stroke impedes post-injury tissue repairment and neurological functional recovery. Developing innovative therapeutic strategies that simultaneously suppress detrimental inflammatory cascades and facilitate neurorestorative processes is critical for improving long-term rehabilitation outcomes.

Methods

We employed a microglia depletion-repopulation paradigm by administering PLX5622 for 7 days post-ischemia; followed by a 7-day withdrawal period to allow microglia repopulation. Single-cell transcriptomics, behavioral testing, cytokine arrays, flow cytometry, and immunofluorescence were used to assess the effects of microglia repopulation and delineate the transition of reshaped immune microenvironment.

Results

PLX5622 a

SUBMITTER: Shi L 

PROVIDER: S-EPMC12423550 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature