Oligotrophic Media Compared with a Tryptic Soy Agar or Broth for the Recovery of <i>Burkholderia cepacia</i> Complex from Different Storage Temperatures and Culture Conditions.

ABSTRACT: The Burkholderia cepacia complex (BCC) is capable of remaining viable in low-nutrient environments and harsh conditions, posing a contamination risk in non-sterile pharmaceutical products as well as a challenge for detection. To develop optimal recovery methods to detect BCC, three oligotrophic media were evaluated and compared with nutrient media for the recovery of BCC from autoclaved distilled water or antiseptic solutions. Serial dilutions (10^-1 to 10^-12 CFU/ml) of 20 BCC strains were inoculated into autoclaved distilled water and stored at 6°C, 23°C and 42°C for 42 days. Six suspensions of Burkholderia cenocepacia were used to inoculate aqueous solutions containing 5 µg/ml and 50 µg/ml chlorhexidine gluconate (CHX) and 10 µg/ml benzalkonium chloride

SUBMITTER: Ahn Y 

PROVIDER: S-EPMC12423783 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature