Optimization of Methodology for Simultaneous Quantification of Trigonelline, 5‑Caffeoylquinic Acid, and Caffeine in Green and Roasted Coffee Extracts by HPLC.

ABSTRACT: The main bioactive compounds in coffee beans affecting beverage quality include trigonelline, 5-caffeoylquinic acid (5-CQA), and caffeine. Despite the use of various analytical techniques, there is a need for a faster and more accessible alternative to the costly and complex methods used in routine coffee composition analysis. This study aimed to optimize and validate a high-performance liquid chromatography with ultraviolet detection (HPLC-UV-DAD) method for the simultaneous quantification of these three bioactive compounds in five Coffea arabica cultivars (Yellow Catuai, Guesha, Arara, Red Catuai, and Laurina). The evaluated parameters included linearity, accuracy, robustness, precision, limit of quantification, and detection. The proposed method met the criteria of the analytical

SUBMITTER: da Silva WB 

PROVIDER: S-EPMC12423835 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature