Non-Invasive Preimplantation Genetic Testing: Cell-Free DNA Detection in Embryo Culture Media Using a Plasmonic Biosensor.

ABSTRACT: In vitro fertilization (IVF) faces challenges in evaluating embryo quality and in determining the genetic health of embryos. Key biomarkers in the culture medium, including nucleic acids and proteins, offer promising avenues for noninvasive assessment. However, small sample volumes, low biomolecule concentrations, and potential contaminants complicate the reliable detection of genetic indicators. In this context, we developed a noninvasive preimplantation genetic testing (niPGT) approach using a superparamagnetic particles-enhanced surface plasmon resonance (SPR) imaging biosensor capable of detecting single-point mutations in nonamplified cell-free DNA released into the spent culture medium during early embryo development. Magnetic beads with a biotinylated locked nucleic acid sequence ca

SUBMITTER: Bellassai N 

PROVIDER: S-EPMC12424020 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature