High-throughput microfluidic real-time PCR as a promising tool in disease ecology.

ABSTRACT: Among recent advances in molecular biology for studying infectious diseases, the microfluidic high-throughput real-time polymerase chain reaction (Htrt PCR) has emerged as an efficient first-line tool for the detection of a wide range of infectious agents (IA) in a host system. This technology allows large batches of samples to be screened simultaneously for tens of targeted IA by real-time PCR. It represents a promising tool in disease ecology. As a proof of concept, we present here the development steps and initial application of a microfluidic Htrt PCR system for the detection of DNA from 28 selected IA in a set of wild vertebrates. We applied this approach to 497 samples (mainly mucosal swabs and necropsy tissues) from 274 seabirds and 80 mammals from Southern Ocean islands. This ecosy

SUBMITTER: Bralet T 

PROVIDER: S-EPMC12424280 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature