The Biosynthetic Pathway to the Pyrroloiminoquinone Marine Natural Product Ammosamide C.

ABSTRACT: Ammosamide C is a marine natural product containing a highly decorated pyrroloiminoquinone core. Studies on the biosynthetic gene cluster (BGC) that produces ammosamides previously revealed that they are made by a series of posttranslational modifications (PTMs). The BGC includes genes encoding a precursor peptide AmmA and four enzymes known as PEptide Aminoacyl-tRNA Ligases (PEARLs). Initial studies into the ammosamide biosynthetic pathway demonstrated Trp addition to a precursor peptide by the PEARL AmmB₂. Thereafter, sequential modifications by several enzymes including two other PEARLs lead to the formation of a peptide intermediate bearing a C-terminal diaminoquinone. In the present work, we present the biosynthetic steps that convert this intermediate to ammosamide C. The

SUBMITTER: Ramos-Figueroa JS 

PROVIDER: S-EPMC12424672 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature